@article {721,
	title = {MALAT-1, a Novel Noncoding RNA, and Thymosin β4 Predict Metastasis and Survival in Early-Stage Non-Small Cell Lung Cancer},
	journal = {Oncogene},
	volume = {22},
	year = {2003},
	pages = {8031-8041},
	abstract = {<p>Early-stage non-small cell lung cancer (NSCLC) can be cured by surgical resection, but a substantial fraction of patients ultimately dies due to distant metastasis. In this study, we used subtractive hybridization to identify gene expression differences in stage I NSCLC tumors that either did or did not metastasize in the course of disease. Individual clones (n 1\&frasl;4 225) were sequenced and quantitative RT\&ndash;PCR verified overexpression in metastasizing samples. Several of the identified genes (eIF4A1, thymosin b4 and a novel transcript named MALAT-1) were demonstrated to be significantly associated with metastasis in NSCLC patients (n 1\&frasl;4 70). The genes\&rsquo; association with metastasis was stage- and histology specific. The Kaplan\&ndash;Meier analyses identified MALAT-1 and thymosin b4 as prognostic parameters for patient survival in stage I NSCLC. The novel MALAT-1 transcript is a noncoding RNA of more than 8000nt expressed from chromosome 11q13. It is highly expressed in lung, pancreas and other healthy organs as well as in NSCLC. MALAT-1 expressed sequences are conserved across several species indicating its potentially important function. Taken together, these data contribute to the identification of early-stage NSCLC patients that are at high risk to develop metastasis. The identification of MALAT-1 emphasizes the potential role of noncoding RNAs in human cancer.</p>
},
	author = {Ji, Ping and Diederichs, Sven and Wang, Wenbing and B{\"o}ing, Sebastian and Metzger, Ralf and Schneider, Paul M. and Tidow, Nicola and Brandt, Burkhard and Buerger, Horst and Bulk, Etmar and Michael Thomas and Wolfgang E. Berdel and Hubert Serve and Carsten M{\"u}ller-Tidow}
}
