01566nas a2200205   4500008004100000020001400041245011300055210006900168260001100237490000800248520092800256653001501184100002001199700001901219700001801238700002301256700002201279700002101301856003801322       2008                        eng d  a0031-900700aTemperature Response of (129)Xe Depolarization Transfer and its Application for Ultrasensitive NMR Detection0 aTemperature Response of 129Xe Depolarization Transfer and its Ap  cJun 270 v1003 a<p>Trapping xenon in functionalized cryptophane cages makes the sensitivity of hyperpolarized (HP) (129)Xe available for specific NMR detection of biomolecules. Here, we study the signal transfer onto a reservoir of unbound HP xenon by gating the residence time of the nuclei in the cage through the temperature-dependant exchange rate. Temperature changes larger than similar to 0.6 K are detectable as an altered reservoir signal. The temperature response is adjustable with lower concentrations of caged xenon providing more sensitivity at higher temperatures. Ultrasensitive detection of functionalized cryptophane at 310 K is demonstrated with a concentration of 10 nM, corresponding to a similar to 4000-fold sensitivity enhancement compared to conventional detection. This makes HPNMR capable of detecting such constructs in concentrations far below the detection limit of benchtop uv-visible light absorbance.</p>
10abiosensors1 aSchroeder, Leif1 aMeldrum, Tyler1 aSmith, Monica1 aLowery, Thomas, J.1 aWemmer, David, E.1 aPines, Alexander  u<Go to ISI>://WOS:000257230500066