02359nas a2200277   4500008004100000245012700041210007000168300001400238490000700252520142000259100001301679700002101692700001801713700002201731700001801753700002401771700001801795700002101813700001901834700001601853700002001869700002501889700001801914700002701932856012201959       2003                        eng d00aMALAT-1, a Novel Noncoding RNA, and Thymosin β4 Predict Metastasis and Survival in Early-Stage Non-Small Cell Lung Cancer0 aMALAT1 a Novel Noncoding RNA and Thymosin β4 Predict Metastasis   a8031-80410 v223 a<p>Early-stage non-small cell lung cancer (NSCLC) can be cured by surgical resection, but a substantial fraction of patients ultimately dies due to distant metastasis. In this study, we used subtractive hybridization to identify gene expression differences in stage I NSCLC tumors that either did or did not metastasize in the course of disease. Individual clones (n 1&frasl;4 225) were sequenced and quantitative RT&ndash;PCR verified overexpression in metastasizing samples. Several of the identified genes (eIF4A1, thymosin b4 and a novel transcript named MALAT-1) were demonstrated to be significantly associated with metastasis in NSCLC patients (n 1&frasl;4 70). The genes&rsquo; association with metastasis was stage- and histology specific. The Kaplan&ndash;Meier analyses identified MALAT-1 and thymosin b4 as prognostic parameters for patient survival in stage I NSCLC. The novel MALAT-1 transcript is a noncoding RNA of more than 8000nt expressed from chromosome 11q13. It is highly expressed in lung, pancreas and other healthy organs as well as in NSCLC. MALAT-1 expressed sequences are conserved across several species indicating its potentially important function. Taken together, these data contribute to the identification of early-stage NSCLC patients that are at high risk to develop metastasis. The identification of MALAT-1 emphasizes the potential role of noncoding RNAs in human cancer.</p>
1 aJi, Ping1 aDiederichs, Sven1 aWang, Wenbing1 aBöing, Sebastian1 aMetzger, Ralf1 aSchneider, Paul, M.1 aTidow, Nicola1 aBrandt, Burkhard1 aBuerger, Horst1 aBulk, Etmar1 aThomas, Michael1 aBerdel, Wolfgang, E.1 aServe, Hubert1 aMüller-Tidow, Carsten  uhttps://dev7.lt.org/malat-1-novel-noncoding-rna-and-thymosin-v4-predict-metastasis-and-survival-early-stage-non-small